čNew Mexico State University, Department of Chemistry and Biochemistry, Las Cruces, NM 88003; and ČLos Alamos National Laboratories, Life Sciences Division, Los Alamos, NM 87545
The uptake and biotransformation of TNT was studied in cell suspension cultures and in whole plants of Datura innoxia and Lycopersicon peruvianum. In cell culture, TNT was rapidly removed from the growth medium and recovered from the cell extract in the form of a variety of biotransformation products resulting from nitroreduction, deamination, N-acetylation and side chain oxidation to aldehyde and carboxylic acid metabolites. Whole plants of the same species grew well in soils contaminated with TNT up to 750 ppm; at 1000 ppm TNT the Datura plants showed some signs of phytotoxicity, while the Lycopersicon plants were severely affected. Both species removed TNT from soil and stored its metabolites at levels up to thirty times higher than the TNT soil concentrations. After a two week growth period, only 4 to 9.2% of the applied TNT was found in the soils.
bioremediation, 2,4,6-trinitrotoluene (TNT) soil, explosives, plants
This paper is from the Proceedings of the 10th Annual Conference on Hazardous Waste Research 1995, published in hard copy and on the Web by the Great Plains/Rocky Mountain Hazardous Substance Research Center.
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