71DIRECT OBSERVATION OF A BIOFILM IN SOIL USING FOURIER TRANSFORM INFRARED SPECTROSCOPY |
D.A. Buttry, S. J. Clay, J.P. Turner, and L.A. Bulla, Department of Chemistry, Department of Civil Engineering, and Department of Molecular Biology,University of Wyoming, Laramie, WY 82070 |
Biofilms produced from bacteria and exo-polysaccharides (EPS) are being investigated as a potential barrier to hazardous materials. With this biofilm, an initially porous soil matrix can become very impermeable, effectively producing a barrier. In this study, fourier transform infrared spectroscopy (FTIR) was used to monitor, in situ, the growth rate of a Beijerinckia indica biofilm in soil at the surface of a germanium internal reflection element (IRE). Production of the biofilm was monitored by observation of absorption bands attributed to exopolysaccharides and proteins.
Key words: biofilm, exopolysaccharides, bacterial adhesion
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