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P27EXAMINING THE FATE OF RELEASED MICROORGANISMS IN SOIL THROUGH A MICROCOSM STUDY |
X. Wu(1), L.C. Davis(1), and L.E. Erickson(2), Biochemistry(1) and Chemical Engineering(2), Kansas State University, Manhattan, KS 66502 |
It is known that the microbial communities in the plant rhizosphere have larger population size and diversity than those of the bulk soil microbial communities. It is one of the assumptions of the plant-based remediation idea that plants participate the degradation indirectly by supporting microorganisms which are able to degrade contaminants, but it has not been shown whether or not the presence of plants would favor the survival and function of introduced microorganisms for the purpose of bioremediation.
A microcosm study has been conducted to investigate the fate of introduced Pseudomonas putida F 1 in intact soil core microcosms with or without plants. P.p.F1 carries a toluene dioxygenase system and can grow on toluene as a sole carbon source and will degrade TCE cometabolically. It may also be useful as an carrier for genes that permit degradation of other contaminants. Microbiological as well as molecular biology based methods including plate counting on selective medium, random primed PCR, and introducing marker genes have been applied or developed in this research. Key words: rhizosphere, bioremediation, Pseudomonas, toluene, trichloroethylene
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